Microbial community structure and functional diversity of nitrogen-fixing bacteria associated with Colophospermum mopane.
Identifieur interne : 000175 ( Main/Exploration ); précédent : 000174; suivant : 000176Microbial community structure and functional diversity of nitrogen-fixing bacteria associated with Colophospermum mopane.
Auteurs : Claudia Sofía Burbano [Allemagne] ; Jann Lasse Grönemeyer [Allemagne] ; Thomas Hurek [Allemagne] ; Barbara Reinhold-Hurek [Allemagne]Source :
- FEMS microbiology ecology [ 1574-6941 ] ; 2015.
Descripteurs français
- KwdFr :
- ADN bactérien (génétique), ARN ribosomique 16S (génétique), Actinobacteria (génétique), Actinobacteria (isolement et purification), Afrique australe (MeSH), Analyse de séquence d'ADN (MeSH), Bacteroidetes (génétique), Bacteroidetes (isolement et purification), Biodiversité (MeSH), Fabaceae (microbiologie), Fixation de l'azote (physiologie), Microbiologie du sol (MeSH), Proteobacteria (génétique), Proteobacteria (isolement et purification), Racines de plante (microbiologie), Sol (MeSH), Séquence nucléotidique (MeSH), Techniques de typage bactérien (MeSH).
- MESH :
- génétique : ADN bactérien, ARN ribosomique 16S, Actinobacteria, Bacteroidetes, Proteobacteria.
- isolement et purification : Actinobacteria, Bacteroidetes, Proteobacteria.
- microbiologie : Fabaceae, Racines de plante.
- physiologie : Fixation de l'azote.
- Afrique australe, Analyse de séquence d'ADN, Biodiversité, Microbiologie du sol, Sol, Séquence nucléotidique, Techniques de typage bactérien.
English descriptors
- KwdEn :
- Actinobacteria (genetics), Actinobacteria (isolation & purification), Africa, Southern (MeSH), Bacterial Typing Techniques (MeSH), Bacteroidetes (genetics), Bacteroidetes (isolation & purification), Base Sequence (MeSH), Biodiversity (MeSH), DNA, Bacterial (genetics), Fabaceae (microbiology), Nitrogen Fixation (physiology), Plant Roots (microbiology), Proteobacteria (genetics), Proteobacteria (isolation & purification), RNA, Ribosomal, 16S (genetics), Sequence Analysis, DNA (MeSH), Soil (MeSH), Soil Microbiology (MeSH).
- MESH :
- chemical , genetics : DNA, Bacterial, RNA, Ribosomal, 16S.
- genetics : Actinobacteria, Bacteroidetes, Proteobacteria.
- isolation & purification : Actinobacteria, Bacteroidetes, Proteobacteria.
- microbiology : Fabaceae, Plant Roots.
- physiology : Nitrogen Fixation.
- Africa, Southern, Bacterial Typing Techniques, Base Sequence, Biodiversity, Sequence Analysis, DNA, Soil, Soil Microbiology.
Abstract
Colophospermum mopane is an indigenous legume tree that grows in Southern Africa and is one of the predominant trees of the woodland vegetation. In order to increase knowledge about its ecology, especially how C. mopane thrives in the nitrogen-poor soils of the region, we analyzed the root-associated bacteria to assess the active diazotrophic diversity and total microbial diversity by culture-dependent and independent techniques. Root nodules were not detected but in some samples the lateral roots showed an outgrowth-like protuberance, that were not likely to have functions related to legume root nodules. The bacterial isolates recovered were related to Actinobacteria, Firmicutes and Proteobacteria. The total microbial diversity was dominated by Actinobacteria-related phylotypes, while the active diazotrophic diversity showed that the majority of the sequences were related to the order Rhizobiales but also to Spirochaetes, Firmicutes, Bacteroidetes and Deltaproteobacteria. Several isolates showed characteristics of plant growth-promoting bacteria. These findings increase the spectrum of possible phylotypes that can be found in legume trees that are typically nodulated by Alpha- and Betaproteobacteria, and reveal for the first time a surprising diversity of nitrogen-fixing bacteria active in legume tree roots.
DOI: 10.1093/femsec/fiv030
PubMed: 25873605
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Box 330440, D-28334 Bremen, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Microbe-Plant Interactions, Center for Biomolecular Interactions Bremen (CBIB), Faculty of Biology and Chemistry, University of Bremen, PO. Box 330440, D-28334 Bremen</wicri:regionArea><placeName><region type="land" nuts="3">Brême (Land)</region><settlement type="city">Brême</settlement></placeName></affiliation></author><author><name sortKey="Gronemeyer, Jann Lasse" sort="Gronemeyer, Jann Lasse" uniqKey="Gronemeyer J" first="Jann Lasse" last="Grönemeyer">Jann Lasse Grönemeyer</name><affiliation wicri:level="3"><nlm:affiliation>Department of Microbe-Plant Interactions, Center for Biomolecular Interactions Bremen (CBIB), Faculty of Biology and Chemistry, University of Bremen, PO. Box 330440, D-28334 Bremen, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Microbe-Plant Interactions, Center for Biomolecular Interactions Bremen (CBIB), Faculty of Biology and Chemistry, University of Bremen, PO. Box 330440, D-28334 Bremen</wicri:regionArea><placeName><region type="land" nuts="3">Brême (Land)</region><settlement type="city">Brême</settlement></placeName></affiliation></author><author><name sortKey="Hurek, Thomas" sort="Hurek, Thomas" uniqKey="Hurek T" first="Thomas" last="Hurek">Thomas Hurek</name><affiliation wicri:level="3"><nlm:affiliation>Department of Microbe-Plant Interactions, Center for Biomolecular Interactions Bremen (CBIB), Faculty of Biology and Chemistry, University of Bremen, PO. Box 330440, D-28334 Bremen, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Microbe-Plant Interactions, Center for Biomolecular Interactions Bremen (CBIB), Faculty of Biology and Chemistry, University of Bremen, PO. Box 330440, D-28334 Bremen</wicri:regionArea><placeName><region type="land" nuts="3">Brême (Land)</region><settlement type="city">Brême</settlement></placeName></affiliation></author><author><name sortKey="Reinhold Hurek, Barbara" sort="Reinhold Hurek, Barbara" uniqKey="Reinhold Hurek B" first="Barbara" last="Reinhold-Hurek">Barbara Reinhold-Hurek</name><affiliation wicri:level="3"><nlm:affiliation>Department of Microbe-Plant Interactions, Center for Biomolecular Interactions Bremen (CBIB), Faculty of Biology and Chemistry, University of Bremen, PO. Box 330440, D-28334 Bremen, Germany breinhold@uni-bremen.de.</nlm:affiliation><country wicri:rule="url">Allemagne</country><wicri:regionArea>Department of Microbe-Plant Interactions, Center for Biomolecular Interactions Bremen (CBIB), Faculty of Biology and Chemistry, University of Bremen, PO. Box 330440, D-28334 Bremen</wicri:regionArea><placeName><region type="land" nuts="3">Brême (Land)</region><settlement type="city">Brême</settlement></placeName></affiliation></author></analytic><series><title level="j">FEMS microbiology ecology</title><idno type="eISSN">1574-6941</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Actinobacteria (genetics)</term><term>Actinobacteria (isolation & purification)</term><term>Africa, Southern (MeSH)</term><term>Bacterial Typing Techniques (MeSH)</term><term>Bacteroidetes (genetics)</term><term>Bacteroidetes (isolation & purification)</term><term>Base Sequence (MeSH)</term><term>Biodiversity (MeSH)</term><term>DNA, Bacterial (genetics)</term><term>Fabaceae (microbiology)</term><term>Nitrogen Fixation (physiology)</term><term>Plant Roots (microbiology)</term><term>Proteobacteria (genetics)</term><term>Proteobacteria (isolation & purification)</term><term>RNA, Ribosomal, 16S (genetics)</term><term>Sequence Analysis, DNA (MeSH)</term><term>Soil (MeSH)</term><term>Soil Microbiology (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN bactérien (génétique)</term><term>ARN ribosomique 16S (génétique)</term><term>Actinobacteria (génétique)</term><term>Actinobacteria (isolement et purification)</term><term>Afrique australe (MeSH)</term><term>Analyse de séquence d'ADN (MeSH)</term><term>Bacteroidetes (génétique)</term><term>Bacteroidetes (isolement et purification)</term><term>Biodiversité (MeSH)</term><term>Fabaceae (microbiologie)</term><term>Fixation de l'azote (physiologie)</term><term>Microbiologie du sol (MeSH)</term><term>Proteobacteria (génétique)</term><term>Proteobacteria (isolement et purification)</term><term>Racines de plante (microbiologie)</term><term>Sol (MeSH)</term><term>Séquence nucléotidique (MeSH)</term><term>Techniques de typage bactérien (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Bacterial</term><term>RNA, Ribosomal, 16S</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Actinobacteria</term><term>Bacteroidetes</term><term>Proteobacteria</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN bactérien</term><term>ARN ribosomique 16S</term><term>Actinobacteria</term><term>Bacteroidetes</term><term>Proteobacteria</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Actinobacteria</term><term>Bacteroidetes</term><term>Proteobacteria</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Actinobacteria</term><term>Bacteroidetes</term><term>Proteobacteria</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Fabaceae</term><term>Racines de plante</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Fabaceae</term><term>Plant Roots</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Fixation de l'azote</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Nitrogen Fixation</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Africa, Southern</term><term>Bacterial Typing Techniques</term><term>Base Sequence</term><term>Biodiversity</term><term>Sequence Analysis, DNA</term><term>Soil</term><term>Soil Microbiology</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Afrique australe</term><term>Analyse de séquence d'ADN</term><term>Biodiversité</term><term>Microbiologie du sol</term><term>Sol</term><term>Séquence nucléotidique</term><term>Techniques de typage bactérien</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Colophospermum mopane is an indigenous legume tree that grows in Southern Africa and is one of the predominant trees of the woodland vegetation. In order to increase knowledge about its ecology, especially how C. mopane thrives in the nitrogen-poor soils of the region, we analyzed the root-associated bacteria to assess the active diazotrophic diversity and total microbial diversity by culture-dependent and independent techniques. Root nodules were not detected but in some samples the lateral roots showed an outgrowth-like protuberance, that were not likely to have functions related to legume root nodules. The bacterial isolates recovered were related to Actinobacteria, Firmicutes and Proteobacteria. The total microbial diversity was dominated by Actinobacteria-related phylotypes, while the active diazotrophic diversity showed that the majority of the sequences were related to the order Rhizobiales but also to Spirochaetes, Firmicutes, Bacteroidetes and Deltaproteobacteria. Several isolates showed characteristics of plant growth-promoting bacteria. These findings increase the spectrum of possible phylotypes that can be found in legume trees that are typically nodulated by Alpha- and Betaproteobacteria, and reveal for the first time a surprising diversity of nitrogen-fixing bacteria active in legume tree roots. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">25873605</PMID><DateCompleted><Year>2015</Year><Month>10</Month><Day>01</Day></DateCompleted><DateRevised><Year>2018</Year><Month>12</Month><Day>02</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1574-6941</ISSN><JournalIssue CitedMedium="Internet"><Volume>91</Volume><Issue>4</Issue><PubDate><Year>2015</Year><Month>Apr</Month></PubDate></JournalIssue><Title>FEMS microbiology ecology</Title><ISOAbbreviation>FEMS Microbiol Ecol</ISOAbbreviation></Journal><ArticleTitle>Microbial community structure and functional diversity of nitrogen-fixing bacteria associated with Colophospermum mopane.</ArticleTitle><ELocationID EIdType="doi" ValidYN="Y">10.1093/femsec/fiv030</ELocationID><ELocationID EIdType="pii" ValidYN="Y">fiv030</ELocationID><Abstract><AbstractText>Colophospermum mopane is an indigenous legume tree that grows in Southern Africa and is one of the predominant trees of the woodland vegetation. In order to increase knowledge about its ecology, especially how C. mopane thrives in the nitrogen-poor soils of the region, we analyzed the root-associated bacteria to assess the active diazotrophic diversity and total microbial diversity by culture-dependent and independent techniques. Root nodules were not detected but in some samples the lateral roots showed an outgrowth-like protuberance, that were not likely to have functions related to legume root nodules. The bacterial isolates recovered were related to Actinobacteria, Firmicutes and Proteobacteria. The total microbial diversity was dominated by Actinobacteria-related phylotypes, while the active diazotrophic diversity showed that the majority of the sequences were related to the order Rhizobiales but also to Spirochaetes, Firmicutes, Bacteroidetes and Deltaproteobacteria. Several isolates showed characteristics of plant growth-promoting bacteria. These findings increase the spectrum of possible phylotypes that can be found in legume trees that are typically nodulated by Alpha- and Betaproteobacteria, and reveal for the first time a surprising diversity of nitrogen-fixing bacteria active in legume tree roots. </AbstractText><CopyrightInformation>© FEMS 2015. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Burbano</LastName><ForeName>Claudia Sofía</ForeName><Initials>CS</Initials><AffiliationInfo><Affiliation>Department of Microbe-Plant Interactions, Center for Biomolecular Interactions Bremen (CBIB), Faculty of Biology and Chemistry, University of Bremen, PO. Box 330440, D-28334 Bremen, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Grönemeyer</LastName><ForeName>Jann Lasse</ForeName><Initials>JL</Initials><AffiliationInfo><Affiliation>Department of Microbe-Plant Interactions, Center for Biomolecular Interactions Bremen (CBIB), Faculty of Biology and Chemistry, University of Bremen, PO. Box 330440, D-28334 Bremen, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hurek</LastName><ForeName>Thomas</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Department of Microbe-Plant Interactions, Center for Biomolecular Interactions Bremen (CBIB), Faculty of Biology and Chemistry, University of Bremen, PO. Box 330440, D-28334 Bremen, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Reinhold-Hurek</LastName><ForeName>Barbara</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Department of Microbe-Plant Interactions, Center for Biomolecular Interactions Bremen (CBIB), Faculty of Biology and Chemistry, University of Bremen, PO. Box 330440, D-28334 Bremen, Germany breinhold@uni-bremen.de.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>04</Month><Day>01</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>FEMS Microbiol Ecol</MedlineTA><NlmUniqueID>8901229</NlmUniqueID><ISSNLinking>0168-6496</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004269">DNA, Bacterial</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012336">RNA, Ribosomal, 16S</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012987">Soil</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D039903" MajorTopicYN="N">Actinobacteria</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000353" MajorTopicYN="N">Africa, Southern</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015373" MajorTopicYN="N">Bacterial Typing Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D041963" MajorTopicYN="N">Bacteroidetes</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D044822" MajorTopicYN="N">Biodiversity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004269" MajorTopicYN="N">DNA, Bacterial</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007887" MajorTopicYN="N">Fabaceae</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009586" MajorTopicYN="N">Nitrogen Fixation</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020560" MajorTopicYN="N">Proteobacteria</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012336" MajorTopicYN="N">RNA, Ribosomal, 16S</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017422" MajorTopicYN="N">Sequence Analysis, DNA</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012987" MajorTopicYN="N">Soil</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012988" MajorTopicYN="N">Soil Microbiology</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">endophytes</Keyword><Keyword MajorTopicYN="N">expression</Keyword><Keyword MajorTopicYN="N">nitrogenase gene</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>03</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>4</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>4</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>10</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25873605</ArticleId><ArticleId IdType="pii">fiv030</ArticleId><ArticleId IdType="doi">10.1093/femsec/fiv030</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li></country><region><li>Brême (Land)</li></region><settlement><li>Brême</li></settlement></list><tree><country name="Allemagne"><region name="Brême (Land)"><name sortKey="Burbano, Claudia Sofia" sort="Burbano, Claudia Sofia" uniqKey="Burbano C" first="Claudia Sofía" last="Burbano">Claudia Sofía Burbano</name></region><name sortKey="Gronemeyer, Jann Lasse" sort="Gronemeyer, Jann Lasse" uniqKey="Gronemeyer J" first="Jann Lasse" last="Grönemeyer">Jann Lasse Grönemeyer</name><name sortKey="Hurek, Thomas" sort="Hurek, Thomas" uniqKey="Hurek T" first="Thomas" last="Hurek">Thomas Hurek</name><name sortKey="Reinhold Hurek, Barbara" sort="Reinhold Hurek, Barbara" uniqKey="Reinhold Hurek B" first="Barbara" last="Reinhold-Hurek">Barbara Reinhold-Hurek</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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